Statement A: Enzymes are biological catalysts and lower the activation energy significantly compared to ordinary acid catalysis. Hence, this statement is correct.
Statement B: During denaturation, hydrogen bonds and other weak interactions responsible for secondary and tertiary structures are broken. However, peptide bonds (primary structure) remain intact. Hence, this statement is correct.
Statement C: Nucleotides are joined by phosphodiester linkage between C₃′ of one sugar and C₅′ of another sugar, not between C₁ and C₄. Hence, this statement is incorrect.
Statement D: Overall folding of a single polypeptide chain represents tertiary structure. Quaternary structure refers to association of multiple polypeptide chains. Hence, this statement is incorrect.
Correct statements: A and B only
Correct Option: D
Updated for JEE Main 2026: This PYQ is important for JEE Mains, JEE Advanced and other competitive exams. Practice more questions from this chapter.